Too Much L Glutamine Cell Culture

The body requires 20 essential and nonessential amino acids.

Too much l glutamine cell culture.

L glutamine is a non essential amino acid that is often simply called glutamine. It is used extensively in wide range of media including classical media serum free media insect cell culture. L glutamine is an amino acid supplement commonly added to mammalian cell culture media. One study used advanced lab techniques to determine how much l glutamine is found in various foods.

Glutamine is an amino acid a building block for proteins found naturally in the body. However of greater physiological importance to many cells l glutamine is a precursor of l glutamate. The center however also notes that research is conflicting in this area and glutamine might also cause more tumors to grow. L glutamine is unstable at physiological ph in liquid media.

It is produced by the body and is also found in food. Powdered glutamine is supplied as a usp grade chemical and liquid formulations of glutamine are supplied at 200 mm 100x at a final concentration of 29 2 mg ml in 0 85 nacl. The following are the percentages of protein made up of l glutamine in each food. L glutamine is supplied in both liquid and powdered forms to be used as an amino acid supplement for cell culture media.

Glutamine supplements may interact with chemotherapy medications making them more effective and helping to reduce their side effects according to the university of maryland medical center. This review will highlight the critical role of l glutamine and l glutamate metabolism for the maintenance and promotion of cell function in a diverse selection of cell types. In cell culture systems l glutamine serves as a crucial component of culture media. Most commercially available media are formulated with free l glutamine which is either included in the basal formula or added to liquid formulations at time of use.